Supplementary Figure 7: 20-Å low-pass-filtered cryo-EM density map of AtOSCA1.1 and single-channel currents of AtOSCA1.1. | Nature Structural & Molecular Biology

Supplementary Figure 7: 20-Å low-pass-filtered cryo-EM density map of AtOSCA1.1 and single-channel currents of AtOSCA1.1.

From: Structure of the mechanosensitive OSCA channels

Supplementary Figure 7

a,b, Top (a) and bottom (b) views of the cryo-EM density map of AtOSCA1.1 with a 20-Å low-pass filter. Monomer A and B are colored in blue and green. The pore regions (M3, M4, M5, M6, and M7) are colored in magenta and indicated with arrows. c–e, Histograms of negative-pressure-activated wild-type AtOSCA1.1 (c), E462K (d), and E462A (e) single-channel currents at a holding potential of –60 mV with 1-s duration. Bin width is 0.05 pA. f, Reversal potential of wild-type AtOSCA1.1, E462K, and E462A in the indicated solutions (n = 3, data are means ± s.e.m.). n stands for the number of independent experiments.

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