Supplementary Figure 6: Pruning of pervasively deposited macroH2A requires active transcription (related to Fig. 5).
From: Transcription-associated histone pruning demarcates macroH2A chromatin domains
(a) Metagene plot showing the occupancy of Pol II S2P (MEF)27 at indicated classes of genes in iDFs. (b) Box plots showing Pol II S2P (MEF)27 density at gene body. Boxes span the lower to upper quartile; median is indicated with a black line; whiskers show locations of the minimum and maximum; P values are calculated using a two-tailed t test, **** P < 2.2 × 10−16. (c) RT-qPCR for indicated genes 18 h after FP treatment. 18 S rRNA used as internal reference. Error bars represent s.d. from n = 2 independent experiments. P values are calculated from two-tailed t test comparing FP treated with untreated (* P < 0.05, ** P < 0.01, *** P < 0.001). (d) Genome browser snapshot showing retention of transient macroH2A2 peaks. (e) Metagene profiles of macroH2A2-GFP relative enrichment at transient or steady-state macroH2A2 peak regions during de novo deposition ±FP. (f) Spearman correlation heatmap and clustering analysis of nChIP-seq profiles genome-wide. Black box highlights clustering of macroH2A2 ChIP profile at 24 h (+FP) with that at 6 h (untreated). (g) Heatmap of macroH2A2-GFP occupancy at inactive gene loci.
