Supplementary Figure 8: H3K27me3 is dispensable for de novo macroH2A2 deposition (related to Fig. 6).
From: Transcription-associated histone pruning demarcates macroH2A chromatin domains
(a) nChIP-qPCR analysis showing the occupancy of H3K27me3 in WT iDFs after FP treatment. Error bars represent s.d. from n = 2 independent experiments. (b) nChIP-qPCR analysis showing H3K27me3 occupancy during de novo macroH2A2-GFP deposition in inducible iDFs. Error bars represent s.d. from n = 2 independent experiments. (c) nChIP-qPCR analysis showing the occupancy of H3K27me3 in inducible iDFs after GSK126 treatment. Error bars represent s.d. from n = 2 independent experiment. (d) Spearman correlation heatmap and clustering analysis of nChIP-seq profiles genome-wide. Black boxes highlight clustering of the 6-h profiles (-/+GSK126) and 24-h profiles (-/+GSK126). (e) Metagene profiles of macroH2A2-GFP occupancy at steady-state or transient macroH2A2 peak regions during de novo deposition after GSK126 pretreatment. (f) Venn diagram showing the overlap of macroH2A2-GFP nChIP-seq peaks after 24 h of dox induction ± pretreatment of GSK126. (g) Example tracks of de novo deposited macroH2A2-GFP after 24 h of dox induction ± GSK126 pretreatment. (h) nChIP-qPCR analysis showing macroH2A2-GFP occupancy after 24 h of dox induction ± pretreatment of GSK126 at regions shown in c. MacroH2A2 ChIP was normalized to H2B. Error bars represent s.d. from n = 2 independent experiment.
