Supplementary Figure 5: CARF localization and its interaction with p32 are RNA and SAMMSON-dependent.
From: SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation
a, p32 (yellow) IF combined with 12S (red) RNA FISH in SK-MEL-28 cells 30 h after transfection with a non-targeting GapmeR (Ctrl), GapmeR3 or GapmeR11. Cell nuclei are stained with DAPI (cyan). Scale bar, 4 μm. Representative image of three independent experiments. b, PLA (cyan) using antibodies against CARF and p32 in SK-MEL-28 cells in normal conditions (Ctrl) or after addition of RNase A. Cell nuclei are stained with DAPI (blue). Scale bar low magnification, 10 μm; high magnification, 2 μm. Representative image of three independent experiments. c, p32 RIP in LCL cells infected with an empty (−) or a SAMMSON-encoding (+) expression vector and western blotting. Representative image of three independent experiments. d, CARF (red) and p32 (yellow) IF in LCL cells as described in c. Cell nuclei are stained with DAPI (blue). Scale bar low magnification, 10 μm, Scale bar high magnification, 2 μm. Representative image of three independent experiments. Uncropped gel images are shown in Supplementary Data Set 1.
