Supplementary Figure 2: Crystallization of Cdc14 with Cbk1 and Sic1 peptides.
From: A PxL motif promotes timely cell cycle substrate dephosphorylation by the Cdc14 phosphatase
(a), Schematic representation of the Cdc141–374 purification scheme, used for the crystallization studies. Purified protein was analyzed by SDS–PAGE followed by Coomassie blue staining. (b), Peptide docking to Cdc14 enhances its thermal stability. Thermal shift assays to determine the Cdc14 melting point (Tm) in the absence or presence of 200 μM Cbk1-derived PxL motif peptide or a scrambled derivative. Means and s.d. from three repeats of the experiment are shown and the melting points are listed together with the coefficient of determination (R2) that indicates the goodness of a fit to a Boltzmann sigmoidal function. (c), Photos of crystals obtained in the indicated space groups of Cdc14 bound by the Cbk1 and Sic1-derived PxL motif peptides.
