Supplementary Figure 6: The Cep3 subunits differ in conformation in the CBF3−CEN3 complex and schematic of the modeled CBF3−Cse4−CEN3 complex.
From: Architecture of the CBF3–centromere complex of the budding yeast kinetochore
a, EM density map from the CBF3–CEN3 DNA complex showing the visible C termini of the two Ndc10 subunits of the Ndc10DBD dimer in close proximity. b, Superimposition of Cep3A and Cep3B of CBF3A from the CBF3–CEN3 DNA complex. CEN3 DNA as bound to Cep3A of CBF3A is shown. The Zn2Cys6 cluster and αMN helix of Cep3A interact with the major grooves of the CCG and TGT motifs of CDEIII, respectively. The major differences between Cep3A and Cep3B involve these structural elements. c, Schematic of Fig. 4b showing the modeled CBF3–Cse4–CEN3 complex. The Cse4 nucleosome is depicted with left-handed chirality. d, Cse4 nucleosome with right-handed chirality. In this instance, the Cse4 nucleosome clashes with CBF3A and therefore represents a less likely scenario. CBF3 is shown transparent.
