Supplementary Figure 7: SEC-MALS analysis of CBF3−CEN3 complexes.
From: Architecture of the CBF3–centromere complex of the budding yeast kinetochore
a, SEC MALS chromatogram for CBF3 complex without CEN3–DNA shows two species of molecular mass 888 kDa and 454 kDa, corresponding to dimeric CBF3 ([Cep3]2–Ctf13–Skp1–[Ndc10]2)2 (expected mass of 453 kDa) and monomeric CBF3 ([Cep3]2–Ctf13–Skp1–[Ndc10]2) (expected mass of 906 kDa). b, In the presence of CEN3 DNA, all CBF3 migrates as a single species of molecular mass 1,018 kDa corresponding to a dimeric CBF3–CEN3 complex ([Cep3]2–Ctf13–Skp1–[Ndc10]2)2–CEN3 (expected mass of 1,010 kDa). The experiments shown were performed in triplicate with similar results.
