Supplementary Figure 1: Biochemical characterization of CBF3−CEN3 complexes.
From: Architecture of the CBF3–centromere complex of the budding yeast kinetochore
a, Left, Coomassie blue–stained SDS–PAGE of CBF3−CEN3 complex. Right, ethidium bromide staining of the same gel. Below, SEC chromatogram of (i) CBF3−CEN3, (ii) CEN3 and (iii) CBF3. b, Left, Coomassie blue–stained SDS–PAGE of CBF3core−Ndc10DBD−CEN3 complex. Right, ethidium bromide staining of the same gel. Below, SEC chromatogram of (i) CBF3core−Ndc10DBD−CEN3, (ii) CEN3 and (iii) CBF3core−Ndc10DBD. c, Mutations of I76 and Y79 of Ctf13 disrupt interactions between CBF3core and Ndc10. The experiments shown were performed in triplicate with similar results.
