Supplementary Figure 5: Mitotic stability assay of wild-type and mutant Ctf13.
From: Architecture of the CBF3–centromere complex of the budding yeast kinetochore
a, Agar plate culture of yeast strains: wild type and mutants in Ctf13, the CCG motif (to AGC) of the CEN3 CDEIII or a combination of Ctf13 and CEN3 mutations. Cultures were incubated for strains harboring mini-chromosome (WT or CEN3 CDEIII CCG mutation) in combination with CTF13 (WT or I76/Y79 mutation) and grown 48 h for about 20 generations of nonselective growth for mini-chromosome. Single colonies were generated on nonselective media and further streaked onto selective media. The percentage of grown colonies on selective media was determined for mitotic transmission of mini-chromosomes. b, Table showing the percentage of colony growth on selective media (% mean survival ± 1 s.d.). Differences in mitotic stability between pairs of strains were analyzed using paired t tests. There was no significant difference between the CTF13I76R/Y79R and CEN3-CDEIIICCG/AGC mutants. The data are based on five replicates.
