Supplementary Figure 3: Local resolution and FSC curve for the ribosome–NatA complex.
From: Ribosome–NatA architecture reveals that rRNA expansion segments coordinate N-terminal acetylation
The ribosome–NatA map (see Supplementary Fig. 2) was further refined focusing on the tunnel exit region to improve the resolution of the bound NatA. a,b, Maps before (a) and after (b) focused refinement are shown colored according to their local resolution. Whereas the overall resolution for the ribosome is below 4 Å, NatA displays a higher degree of flexibility indicated by a gradient of local resolution ranging from 7 to >12 Å. Focused refinement on NatA improved the resolution for the ligand to below 10 Å and an average resolution of 8 Å. c,d, FSC curves for the maps shown in a and b, respectively.
