Supplementary Figure 1: Biochemical characterization of S. cerevisiae III2IV2 SC.
From: Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1
a, UV-visible redox spectrum of the purified yeast III2IV2 SC. Peaks are labeled and correspond to the A-type hemes present in CIV (445/604 nm) and the B- and C-type hemes present in CIII (432/562 nm and 554 nm). b, Gel filtration profile of the yeast III2IV2 SC. The fraction eluting between 12 and 12.5 mL was used for cryo-EM. c, BN-PAGE gel of the purified yeast III2IV2 SC. d, Oxygen consumption rate of the III2IV2 SC (red line) in the presence of 50 μM cytochrome c, initiated by addition of 40 μM decylubiquinol (decylUQH2). A baseline (green) is recorded after addition of 1 mM KCN, an inhibitor of CIV. e, Oxygen consumption rate of the III2IV2 SC without exogenous cytochrome c (experiment details are as in d). Data in a–c are representative of three independent experiments with similar results.
