Supplementary Figure 2: Cryo-EM data collection and workflow of 3D classification and focused refinement.
From: Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1
a, A representative cryo-EM micrograph of III2IV2 SC (scale bar, 30 nm). b, Representative 2D class averages of the III2IV2 SC (scale bar, 20 nm). c, Fourier shell correlation (FSC) curves between two independently refined half-maps for the III2IV2 SC map and for the two CIV maps, each individually refined. d, Surface rendering maps colored according to local resolution. Upper and middle panels present the maps of the III2IV2 SC and CIVa and CIVb after focused refinement. The lower display shows the central sections of the two CIV maps to emphasize further the improvement in resolution (scale bar, 50 Å). e, Euler angle distributions of the 44,915 particles included in the calculation of the III2IV2 SC map for two orientations rotated by 90 degrees. Data collection and structure calculation are not repeated.
