Supplementary Figure 5: E2-mediated architectural changes on chromosome 21 detected by proximity-based-ligation approaches.
From: Phase separation of ligand-activated enhancers licenses cooperative chromosomal enhancer assembly
a. A/B compartments (top panel) and TAD boundaries (bottom panel) calculated from the Hi-C data indicating that these genomic compartments are unaffected by E2 treatment b. Contact map from Hi-C data, analyzed at a resolution of 1 Mb, indicating the presence of very long-distance interactions (enriched contacts) as well as the expected short-range interactions c. 4C-seq revealing TFF1e interactions with the DSCAM-AS1 enhancer region, using TFF1e as a view point, based on analysis of data after excluding all interactions at less than 2 Mb to permit quantitative analysis of interactions between these loci separated by >2 Mb. GRO-seq data of locus is given as a reference.
