Supplementary Figure 7: RNA FISH data showing fraction of cells actively transcribing E2 targets.
From: Phase separation of ligand-activated enhancers licenses cooperative chromosomal enhancer assembly
a. RNA FISH image using intronic and exonic probe against TFF1 mRNA in MCF7 cells treated 15 min with E2. Pooled data from total of > 200 nuclei in replicate. The fraction of cells expressing or not expressing exonic probe is indicated b. RNA FISH image using intronic and exonic probe against NRIP1 mRNA in MCF7 cells treated 15 min with E2. Pooled data from total of > 200 nuclei in replicate. The fraction of cells expressing or not expressing exonic probe is indicated c. Percent of cells (E2-treated, 15 min) expressing NRIP1 or TFF1 by intronic RNA FISH probes, expressing 0, 1, or 2 alleles d. Intensity vs. distance plot of all examined RNA FISH signal using NRIP1 intronic probe in relation to the TFF1 probe (A.U. = Arbitrary Unit) e. Intensity vs. distance plot of all examined RNA FISH signal using DOPEY2 intronic probe in relation to TFF1 probe.
