Supplementary Figure 6: Effect of salt and other challenges on MEG-3 and PGL-3 condensates.
From: A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos
a Representative images of MEG-3 or MEG-3-IDR and PGL-3 co-assemblies in condensation buffer or buffer with 1M NaCl for 15 min as described in Fig. 5a. Scale bar is 5 µm. b Representative images of MEG-3 and PGL-3 co-assemblies after incubation with 1M NaCl for 15 min as described in Fig. 5a. Scale bar is 2 µm. c Graphs showing the relative proportion of MEG-3 in condensates [calculated as the ratio of fluorescence in condensates (IC) over total fluorescence (IT)] on a log scale. Single condensates are in black and co-condensates are in green. Each data point represents the average from 4 images collected in one experiment. For data points marked by ‘*’, no condensates were detected. Bars represent the mean ± SD. Condensates were incubated for 15 min before challenge with the indicated additive for 15 minutes (7 mM ATP/Mg, 5% 2,5 or 1,6 Hexanediol, 0.5% SDS). d Graphs showing the relative proportion of PGL-3 in condensates determined as described in (c).
