Supplementary Figure 7: RNA dependence of MEG-3 and PGL-3 co-condensate formation.
From: A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos
Representative images of MEG-3 and PGL-3 co-assemblies in condensation buffer. All reactions contained 1.8 µM PGL-3 with varying concentrations of MEG-3 on the y-axis and varying concentration of total embryonic RNA on the x-axis. Reactions were incubated for 15 min. Scale bar is 10 µm. Red boxes indicate conditions where both MEG-3 and PGL-3 form round co-condensates. Conditions that included no RNA or low concentrations of RNA (panels on the left) resulted in the co-precipitation of MEG-3/PGL-3 into aggregates of various shapes. Addition of moderate concentrations of RNA induced the formation of round PGL-3/MEG-3 co-condensates. Higher concentrations of RNA were required to induce round condensates with increasing concentrations of MEG-3. The highest concentrations of RNA (≥500 ng/uL, right most panels) seemed to interfere with condensation.
