Supplementary Figure 1: P granule proteins exhibit distinct dynamics.

a Graph showing MEG-3::GFP (n = 22) and PGL-3::mCherry (n = 12) fluorescence recovery after photobleaching (FRAP) in embryos. Granule intensity was measured every 5 s for 300 s before and after bleaching. Values were normalized to initial fluorescence intensity and plotted as an average from multiple embryos. Error bars represent mean ± SEM. Representative images from FRAP experiments are shown below the graph. b Graph showing GLH-1::GFP (n = 9), LAF-1::GFP (n = 7), or DEPS-1::GFP (n = 7) FRAP in arrested oocytes. Granule intensity was measured every 3 s for 180 s (GLH-1, LAF-1) or every 5 s for 300 s (DEPS-1) before and after bleaching. Values were normalized to initial fluorescence intensity and plotted as an average from multiple embryos. Error bars represent mean ± SEM. Representative images from FRAP experiments are shown below the graph. c Graph showing MEG-3::GFP (n = 10) or PGL-3::mCherry (n = 8) FRAP in arrested oocytes. Granule intensity was measured every 5 s for 300 s before and after bleaching. Values were normalized to initial fluorescence intensity and plotted as an average from multiple oocytes. Error bars represent mean ± SEM. Representative images from FRAP experiments are shown below the graph. d Graph showing MEG-3::mCherry (n = 6) fluorescence recovery after photobleaching (FRAP) in embryos. Granule intensity was measured every 7 s for 300 s before and after bleaching. Values were normalized to initial fluorescence intensity and plotted as an average from multiple embryos. Error bars represent mean ± SEM. Representative images from FRAP experiments are shown below the graph. e Graph showing MEG-3::mCherry (n = 3) or PGL-1::GFP (n = 7) FRAP in arrested oocytes. Granule intensity was measured every 5 s for 300 s before and after bleaching. Values were normalized to initial fluorescence intensity and plotted as an average from multiple oocytes. Error bars represent mean ± SEM. Representative images from FRAP experiments are shown below the graph. f Photomicrographs of 4-cell embryos expressing MEG-3::GFP ± mbk-2 RNAi. Scale bar is 10 μm. Note that P granules do not localize properly in embryos depleted of MBK-2 kinase by RNAi. g Photomicrographs of embryos of the indicated genotypes expressing PGL-1::GFP. PGL-1::GFP ± mbk-2 RNAi photomicrographs are of 4-cell embryos. PGL-1::GFP, meg-3meg-4 photomicrograph is of a 2-cell embryo. Scale bar is 10 μm. Note that P granules do not localize properly in embryos depleted of MBK-2 kinase by RNAi or derived from mothers mutant for meg-3 and meg-4. h Graphs showing MEG-3::GFP FRAP in embryos ± mbk-2(RNAi) (n = 21). Error bars represent mean ± SEM. MEG-3 becomes less dynamic in embryos lacking MBK-2. Representative images from FRAP experiments are shown below the graph. i Graphs showing PGL-1::GFP FRAP in embryos ± mbk-2(RNAi) (n = 11). Error bars represent mean ± SEM. PGL-1 dynamics are not affected in embryos lacking MBK-2. Representative images from FRAP experiments are shown below the graph. j Graphs showing PGL-1::GFP FRAP in wild-type and embryos lacking meg-3meg4 (n = 10). Error bars represent mean ± SEM. PGL-1 dynamics increase in meg-3meg-4 embryos. Representative images from FRAP experiments are shown below the graph.