Supplementary Figure 1: EM analysis of MukBEF. | Nature Structural & Molecular Biology

Supplementary Figure 1: EM analysis of MukBEF.

From: A folded conformation of MukBEF and cohesin

Supplementary Figure 1

a, Negative stain 2D class averages for the folded conformation of native E. coli MukBEF using a circular mask of 640 Å. b, SDS-PAGE analysis of purified Desulfovermiculus halophilus MukBEF. The gel was stained with Coomassie. c, Cartoon of intermediate particle shapes of D. halophilus MukBEF indicating the presence of a coiled-coil elbow in different conformations. d, Cryo-EM imaging of D. halophilus MukBEF in unsupported vitreous ice. Contrast was enhanced by use of a Volta phase plate and high total electron dose. Typical fields of view are shown on the left, examples of single particle images are shown on the right. We estimate that approximately 35 % of particles may adopt a fully folded conformation under the conditions used. Low particle abundance and sample heterogeneity prevented further structural analysis.

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