Supplementary Figure 10: The binding of LIN28A and IGF2BP3 to their target RNAs is influenced by m6A modification.
From: RNA structure maps across mammalian cellular compartments
a,b, RNA pull-down assays and RBP Western blots using RNA probes that contain unmodified A, m6A, and U mutation respectively from (a) transcripts IGF2BP3 binds to, and (b) LIN28A binds to m6A sites are marked with red ‘m’. Histograms show RNA pull down with three replicates. Western blots are done with (a) anti-IGF2BP3 antibody or anti-LIN28A antibody, after RNA pull down. The error bars represent standard deviation of replicates. c, Overlap of Lin28a binding sites (including both in Mettl3 knock-out (KO) and in wild-type (WT) mES cells) and m6A sites. For those overlapped sites, Lin28a binds more strongly at 45 out of 68 sites in Mettl3 KO, versus 23 out of 68 sites in WT mES cells.
