Supplementary Figure 4: Workflow of the data processing of the AcrVA5-acetylated MbCas12a in complex with crRNA.
From: An anti-CRISPR protein disables type V Cas12a by acetylation
a, A representative cryo-EM image of MbCas12a-crRNA complex. b, Representative class averages of 2D classification of auto-picked particles. Particles included for further processing were indicated by red boxes. c, Workflow of the image processing, including unsupervised and supervised 3D classification, 3D refinement and CTF refinement. See more details in Methods.
