Supplementary Figure 1: 13C and 15N CP-MAS NMR spectra of low-pH glucagon fibrils.
From: The peptide hormone glucagon forms amyloid fibrils with two coexisting β-strand conformations
The spectra show narrow linewidths and peak doubling, indicating the coexistence of two well-ordered molecular conformations. a, 13C spectra. b, 15N spectra. Peak assignments obtained from 2D and 3D experiments are shown for samples 1–4. All spectra were collected on an 800 MHz spectrometer under 10–14 kHz MAS at a setpoint temperature of 273–293 K. Peaks assigned to fewer than three residues are labeled in samples 7c–9c.
