Supplementary Figure 3: SEC-SAXS studies on TcdB holotoxin at pH 7.4 and 5.0.
From: Structure of the full-length Clostridium difficile toxin B
(a, b) Rg (blue) and I(0) (orange) plots for SEC-SAXS at pH 7.4 and 5.0. (c) SAXS profile at pH 7.4 (blue) and pH 5.0 (orange) were superimposed with a curve fitting using the crystal structure of TcdB holotoxin (black). (d) Pairwise distribution function, P(r), of TcdB holotoxin at pH 7.4 (blue) and 5.0 (orange). (e) The maximum dimension of TcdB holotoxin at acidic pH and the core of TcdB composed of GTD, CPD, and DRBD.
