Supplementary Figure 6: Effects of remodeler depletion on TSS selection.
From: Opposing chromatin remodelers control transcription initiation frequency and start site selection
a, Snapshot of genomic region showing 5’-RACE signal for the Watson (w) and the Crick (c) strands as well as nucleosome occupancy in the presence and absence of RSC; upon RSC depletion the RPO49 gene transcription initiates more upstream comparing to wild-type conditions. b, As in (a) but for SWI-SNF depletion; upon SWI-SNF depletion the SNQ2 gene transcription initiates more frequently downstream comparing to wild-type conditions; additionally, there is more initiation events in the opposite strand just downstream from the upstream-most SNQ2 TSS. c, As in (a) but for ISW2 and INO80 simultaneous depletion; upon “pullers” depletion the FRP6 gene transcription initiates at a downstream position comparing to wild-type conditions. d, Average 5’-RACE signal at genes upregulated upon simultaneous depletion of ISW2 and INO80. e, As in (d) but for downregulated genes.
