Supplementary Figure 1: Auxin-mediated degradation of Spt16 in G2–M arrested cells. | Nature Structural & Molecular Biology

Supplementary Figure 1: Auxin-mediated degradation of Spt16 in G2–M arrested cells.

From: FACT mediates cohesin function on chromatin

Supplementary Figure 1

Cells carrying either SMC1-6HA or SMC1-6HA SPT16-AID (which allows auxin-mediated degradation of Spt16) were arrested in metaphase (G2–M-nocodazole) and exposed to auxin to degrade Spt16. Nuclear and cellular morphology was used following auxin addition to confirm that the cell population remained arrested in metaphase (top). Western analysis for Spt16 confirms its degradation in SPT16-AID cells upon auxin addition (bottom). The bar graph data show the mean and error bars the s.d. of three independent experiments.

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