Supplementary Figure 2: Auxin-mediated degradation of Sth1 in G2–M arrested cells. | Nature Structural & Molecular Biology

Supplementary Figure 2: Auxin-mediated degradation of Sth1 in G2–M arrested cells.

From: FACT mediates cohesin function on chromatin

Supplementary Figure 2

Cells carrying either SMC1-6HA or SMC1-6HA STH1-AID (which allows auxin-mediated degradation of Sth1) were arrested in metaphase (G2–M-nocodazole) and exposed to auxin to degrade Sth1. Western analysis for Sth1 confirms its degradation in STH1-AID cells upon auxin addition.

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