Extended Data Fig. 3: Assessment of BiPK294F affinity for CH1 in presence of nucleotides.
From: UPR proteins IRE1 and PERK switch BiP from chaperone to ER stress sensor
BiPK294F has same binding affinity for CH1, in different nucleotide bound states, as BiPWT. (a) MST profile measuring the binding affinity between BiP and BiPK294F for CH1. (b) same as (a), but in the presence of ADP. (C) same as (a), but with ATP. The experiments indicate that the mutation had no effect on BiP interaction with misfolded substrate protein or affected BiP nucleotide bound conformations. Statistics as in Fig. 4, source data available online.
