Extended Data Fig. 7: Cryo-EM analysis of the tetrameric complex.
From: Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution
a, Summary of single-particle image analysis procedure. b, A representative micrograph. Scale bar, 20 nm. The dimensions of magnified images are 414 Å (width) by 414 Å (height). c, Examples of selected 2D class averages. The box dimensions are 460 Å (width) by 460 Å (height). d, Particle orientation distribution. e, Fourier shell correlation (FSC). Blue line, corrected masked FSC. Solid black line, unmasked FSC. f, Local resolution map. g, Example images of particles larger than the tetramer. The leftmost image shows a 2D projection (side view with the longest width) of the 3D reconstruction of the tetrameric TOM complex. The other images show examples of large particles on micrographs. Estimated oligomeric states are indicated. Scale bar, 100 Å.
