Extended Data Fig. 2: Cryo-EM map and atomic model quality of the dimeric TOM complex.
From: Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution
a, Local resolution represented by a heat map on the density contour (unsharpened, summed map). b, FSC between the EM map and the atomic model. Blue curve, FSCwork (FSC between half map 1 and a model refined against half map 1). Red curve, FSCfree (FSC between half map 2 and the model refined against half map 1). Black curve, FSCfull (FSC between the combined map and the final atomic model refined against the combined map. All refinements were performed by Phenix with the same weight. c, Examples of the density map and the atomic model for indicated segments. Numbers in the brackets indicate ranges of amino acid residues shown. d, Structural comparison of Tom40 and VDAC. Structures of Tom40 (this study; green) and murine VDAC (PDB 3EMN; magenta) are superimposed. Left, view from cytosol. Right, side view. e, Density features (green) for the C-terminal tails of Tom40 are shown in a 5-Å low-pass-filtered map. Shown is vertical cross-section along the Tom40 pores. A weak connection (not shown) between the density in green and α3 of Tom40 is indicated by a dashed line.
