Extended Data Fig. 3: Cryo-EM data processing scheme.
From: InsP6 binding to PIKK kinases revealed by the cryo-EM structure of an SMG1–SMG8–SMG9 complex
Cryo-EM data processing scheme. The 849,831 particles from the 2D classification were initially 3D-classified into five classes. The two classes containing the best aligning particles were combined and auto-refined in RELION to a resolution of 3.45 Å. The dataset was further classified for the C-terminus of the SMG8 protein in cryoSPARC, using heterogeneous refinement. A class-showing density for the SMG8 C-terminal region was then selected and refined using homogenous refinement to a resolution of 3.57 Å.
