Extended Data Fig. 1: CryoEM processing flow chart (Chi-containing substrate).
From: A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair
a, Scheme overview of the cryoEM processing steps for RecBCD with the Chi-containing substrate. Masks used during 3D classifications are shown in green. To display the separation of particles with ordered and disordered Chi sequences, 2D central slices are displayed of each respective masked 3D model. Final maps are coloured by local resolution as estimated by ResMap40. The scale bar shows the colour scale with resolution in Å. b, Upper, an example of a typical micrograph showing particle distribution. Lower, a selection of 2D class averages representing different particle views. c, ‘Gold standard’ FSC curve showing estimated global resolution of the three structures.
