Extended Data Fig. 4: CryoEM processing flow chart (minus 2 substrate).
From: A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair
a, Scheme overview of the cryoEM processing steps for the RecBCD with the Chi-minus 2 substrate (a 3’-tail contained Chi with 6 base T before Chi and 4 base T after Chi, the rest are the same as Chi-containing or Chi-minus substrates). Masks used during 3D classifications are shown in green. Classification with a mask around Chi showed one class with no Chi density as indicated in the displayed 2D central slice of the masked 3D model. The final map is coloured by local resolution as estimated by ResMap45. The scale bar shows the colour scale with resolution in Å. b, Upper, an example of a typical micrograph showing particle distribution. Lower, a selection of 2D class averages representing different particle views. c, ‘Gold standard’ FSC curve showing estimated global resolution of the structure.
