Extended Data Fig. 2: Human SERINC5 purification and EM.
From: A bipartite structural organization defines the SERINC family of HIV-1 restriction factors
a, Size exclusion chromatography profile. b, Left, SDS-PAGE analysis of resulting fractions; right, cleavage of the TwinStrep tag and deglycosylation (uncropped gel images are shown in the Source Data). c, Sample micrograph of negatively stained particles. d, Representative 2D class averages. e, Schematic of image processing and reconstruction of the human SERINC5 cryo-EM structure. Details are given in Extended Methods. f, Left, Gold standard FSC curve for the cryo-EM reconstruction of SERINC5. Right, Euler angle distribution plot for particles included in the final 3D reconstruction; 3DFCS reports a sphericity of 0.976. g, The map colored according to local resolution estimated with blocres.
