Extended Data Fig. 4: Structural comparisons of mouse PRC and NFC.
From: Cutting antiparallel DNA strands in a single active site
a, Cryo-EM structures of NFC with DNA1 (green) and DNA2 (blue) are superimposable. b, Comparison of mouse PRC and NFC structures. Superposition of cryo-EM PRC (red) and NFC (DNA1) (green) structures reveals limited NBD and nonamer movement, which is marked with blue dashed circle (right panel). c, Superposition of crystal (grey) and cryo-EM (red) PRC structures reveals the different NBD and nonamer region (circled in red dashes) due to crystal-lattice contacts. d, e, The zoom-in views of the active center and DNA distortions in the superimposed structures shown in a-b. The catalytic DDE motif and two metal ions (a and b) are labeled; the heptamer of RSS DNA is shown in detailed cartoon presentation; the scissile phosphate of top strand is marked by a large ball. In panel d, bases forming the DNA zipper are labeled. f, A zoom-in view of boxed area in panel b. RAG2 interacts with the minor groove in PRC or the major groove in NFC.
