Extended Data Fig. 5: Structure determination of mouse WT NFC with DNA0 by cryo-EM.
From: Cutting antiparallel DNA strands in a single active site
a, Flow chart for cryo-EM data processing of WT mRAG complexed with DNA0. Data processing was done using RELION. The 3.6Å map labeled in red was used for final model building. b, A surface presentation of the 3.6 Å map of WT NFC (DNA0). Colors are according to the local resolution estimated by ResMap, and the color scale bar is shown on its right. c, Angular distributions of all particles used for the final three-dimensional reconstruction. d, The FSC curves of WT NFC (DNA0) map. The “gold standard” FSC between two independent halves of the map indicates an overall resolution of 3.6 Å. e–h, Representative regions of the map (transparent grey surface). The refined zipper DNA fits the map better (e) than the melted DNA (PDB: 6DBR) (f).
