Extended Data Fig. 8: Remodeling of the active site in NFC and HFC.
From: Cutting antiparallel DNA strands in a single active site
a, Repositioning of αX in the RNH domain during PRC to NFC transition (both are cryo-EM structures). E962 is far from the active site in PRC (light blue) but is positioned for catalysis in NFC (green). b, Anomalous X-ray scattering of the PRC crystals confirms that one Mn2+ and one Zn2+ are bound to each RAG1 subunit. The anomalous map is contoured at 3σ in red. The blue 2Fo-Fc map (contoured at 1σ) highlights R848, which is buried in the minor groove. c, The reconfigured E962 in HFC (pink) after the first DNA cleavage by nicking (green).
