Extended Data Fig. 3: CryoEM processing and reconstruction quality of Mec1-Ddc2. | Nature Structural & Molecular Biology

Extended Data Fig. 3: CryoEM processing and reconstruction quality of Mec1-Ddc2.

From: Mechanism of auto-inhibition and activation of Mec1ATR checkpoint kinase

Extended Data Fig. 3

a, b, Processing tree resulting reconstructions of Mec1-Ddc2 and a second reconstruction in complex with AMP-PNP (see Methods for details). c, f, Local resolution estimates from ResMap, with slice through the density to show internal features, of apo (c) and bound with AMP-PNP (f). d, g, Angular distribution from CisTEM autorefine, and (e, h) Gold-standard Fourier shell correlation (FSC) from RELION-3.0 and CisTEM.

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