Extended Data Fig. 5: CryoEM processing and reconstruction quality of Mec1(F2244L)-Ddc2.
From: Mechanism of auto-inhibition and activation of Mec1ATR checkpoint kinase
a, Processing tree resulting in high resolution reconstructions of Mec1(F2244L)-Ddc2 in complex with AMP-PNP and magnesium captured in two states (see Methods). b, Local resolution estimates from ResMap, with slice through the density to show internal features, of State I, (c) angular distribution from CisTEM autorefine, and (d) Gold-standard Fourier shell correlation (FSC) from RELION-3.0 and CisTEM. e, Local resolution estimates from ResMap of State II, with (f) angular distribution from CisTEM autorefine, and (g) Gold-standard Fourier shell correlation (FSC) from RELION-3.0 and CisTEM.
