Extended Data Fig. 1: Generation of epitope-tagged Tet2 alleles and CLIP quantification.
From: TET2 chemically modifies tRNAs and regulates tRNA fragment levels
a, Genotype validation for 6xHis–HA knock-in at the Tet2 locus by Sanger sequencing. The targeted allele scheme (top), expected protein and DNA sequence (middle), and sequencing traces (bottom) for the two clones used in subsequent experiments are shown. b, Quantification of Fig. 1D; fluorescence signal (crosslinked RNA) was normalized to WB signal (protein). Bars represent the mean + s.e.m. P-value is from a Student’s t-test.
