Extended Data Fig. 9: Conformation of the nucleosomal DNA in the cryo-EM structure of RSC^SAR-nucleosome (ADP-BeF₃).

a, The models of Snf2 (PDB 5Z3U) and RSC^SAR were aligned. A ribbon representation of the nucleosomal DNA is shown for Snf2 (grey) and Sth1 (colored by SHL). Super Helical Locations for the aligned models are shown inside the cryo-EM density of RSC^SAR. b, RSC^SAR with a canonical conformation of the nucleosome. Top panel shows map and model from the initial 3.9 Å consensus refinement (Extended Data Fig. 2b). The nucleosomal DNA is colored purple and basic residues in the N-lobe are colored blue. c, RSC^SAR with a peeled conformation of the nucleosomal DNA exit site. Top panel shows map and model from the 4.3 Å refinement of a subset of particles with a peeled DNA conformation (Extended Data. Fig. 2c). The nucleosomal DNA is colored orange and basic residues in the N-lobe are colored blue. d, Alignment of canonical and peeled DNA structures. Note that basic residues in the N-lobe appear to make contact with the exit site DNA in the peeled conformation. e, SHL6 shifts between the canonical and peeled DNA conformations.