Extended Data Fig. 1: Cryo-EM structure determination of the Sth1-Arp7-Arp9-Rtt102 RSC subcomplex (RSCSAR).
From: Structural insights into assembly and function of the RSC chromatin remodeling complex
a, Workflow for cryo-EM data acquisition and structure determination. Our processing only revealed density for the ARP module. b, Schematic for model building using Coot and Rosetta. Chains from PDB 5TGC and PDB 4I6M were used as a starting model. c, The top 10 Rosetta models were deposited as PDB 6VZG and the top model is shown with its ribbon thickness and color indicating the RMSD among all 10 models. d, The ARP module form SWI/SNF (left) has a single, unbroken α-helix for the HSA domain while that of RSC (right) comprises two α-helices separated by a loop. e, Our cryo-EM has density for a known ATP binding site in Arp7. This site is catalytically dead and the ATP is likely from endogenous ATP pools.
