Extended Data Fig. 2: Cryo-EM structure determination of RSCSAR bound to the nucleosome.
From: Structural insights into assembly and function of the RSC chromatin remodeling complex
a, Workflow for cryo-EM data acquisition and structure determination. b, 3D classification with alignment was used to find ~300,000 particles with better ARP module density. This dataset reached a final global FSC resolution of ~3.9 Å with a local resolution range of 3.4-15 Å. c, Partial signal subtraction followed by 3D classification without alignment was used to identify a sub-set of particles with an alternate DNA conformation at the nucleosome DNA exit site.
