Extended Data Fig. 6: Global and local resolution of isolated RS1.
From: Structures of radial spokes and associated complexes important for ciliary motility
a, FSC curves calculated between masked independent half maps for isolated RS1. Left panel, FSC curves are shown for the consensus refinement of isolated RS1, focused refinement of the stalk, and focused refinement of the spoke head after applying C2 symmetry. Right panel, FSC curves for focused refinements of three subdomains of a single lobe of the RS1 spoke head. The colors of the curves match the masks used in Extended Data Fig. 5g. The nominal resolution was estimated using the FSC=0.143 criterion (dashed line). b, Density maps for the consensus refinement of isolated RS1 and various focused refinements colored by local resolution. The local resolution is colored from 3 to 7 Å.
