Extended Data Fig. 4: Characterization of isolated and induced neurons.
From: TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS
a, Western blotting analysis of isolated primary mouse neurons and N2a cells by probing both pan-neuron (Tuj1) and mature neuron (SYN1 and MAP2) markers. The non-neuronal MEF cells served as control. b, Immunostaining with Tuj1 (red) and SYN1 (green) on isolated primary mouse neurons. Scale bar: 100 μm. c,d,e, Characterization of iPSCs with the stem cell markers Oct-4A and SOX2 (c), induced NPCs with Nestin, Vimentin, SOX2, and Pax6 (d), and differentiated neurons with Tuj1, SYN1, and MAP2 (e). Scale bar: 100 μm. For a-e, a representative example of three independent experiments is shown. Uncropped images for panel a are available as source data online.
