Extended Data Fig. 5: Partial purification of TDP-43 aggregates for global analysis.
From: TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS
a, The fractionation scheme for purifying TDP-43 aggregates. b, and c, Western blotting analysis of TDP-43 in N2a cells in response to sodium arsenite (b) and heat shock (c). d, and e, Western blotting analysis of TDP-43 in soluble and pellet fractions of two indicated neuronal (d) and two non-neuronal (e) cells. (f) Reproducibility of duplicated small RNA-seq on total (left) versus pellet fraction (right) miRNAs. g, RT-qPCR analysis of TDP-43 target and non-target miRNAs in the pellet. Data in g are shown as mean ± s.d. (n = 3 independent experiments). For b-e, a representative example of three independent experiments is shown. Data for graph in g are available as source data online. Uncropped images for panels b-e are available as source data online.
