Extended Data Fig. 7: Mimicking mitochondrial imbalance by RNAi and overexpression.
From: TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS
a, Overexpression of myc-tagged CTF35 in N2a cells, validated with the anti-myc antibody. GAPDH served as loading control. b, Western blotting validation of the siRNA effects on indicated nDNA-encoded mitochondrial proteins in N2a cells. c, Western blotting validation of the efficacy and specificity in double siRNA-treated N2a cells. d, and e, Western blotting of single and double expression of indicated nDNA-encoded mitochondrial proteins. f, Western blotting analysis of the effect of combinatory siRNA treatment (LRPPRC) and overexpression (Bcl2l2) of separate nDNA-encoded mitochondrial proteins in N2a cells. g, Quantification of ROS production upon knockdown of two representative “up-regulated” genes (Fam173b and Timm9; ATP5b as a positive control) or overexpression of two representative “down-regulated” genes (Ndufa9 and ATP5C1; Bcl2l2 as a positive control). Data in g were presented as mean ± s.d. (n = 3 independent experiments). The P values are based on a two-tailed unpaired t test: **p < 0.01, ***p < 0.001. For a-f and g (lower panel), a representative example of three independent experiments is shown. Data for graphs in g are available as source data online. Uncropped images for panels a-f and g are available as source data online.
