Extended Data Fig. 1: Transcriptomic comparison between TDP-43 knockdown and expression of the aggregation-prone CTF35.
From: TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS
a, Confirmation of the knockdown effect of multiple siRNAs against TDP-43 in N2a cells by Western blotting analysis. GAPDH served as loading control. The targeting regions in mouse TDP-43 are indicated with individual siRNAs. Note that siTDP-43-2290 corresponds to the siRNA against TDP-43-3′UTR. For RNA-seq analysis, total RNAs were extracted from N2a cells transfected with siTDP-43-1078 or control siRNA. b, Expression of wt TDP-43 or CTF35 in N2a cells depleted of endogenous TDP-43, analyzed by Western blotting. c, Reproducibility of duplicated RNA-seq libraries from siNC-treated (left) or siTDP-43 treated (right) N2a cells. d, Reproducibility of duplicated RNA-seq libraries from TDP-43 depleted N2a cells complemented with either wt full-length TDP-43 (left) or with CTF35 (right). e, Percentages of commonly affected genes in siTDP-43 treated N2a cells relative to three different sources of ALS patients. For a and b, a representative example of three independent experiments is shown. Uncropped images for panels a and b are available as source data online.
