Extended Data Fig. 6: CTCF loss does not measurably alter histone modification at promoters and enhancers.
From: Promoter-proximal CTCF binding promotes distal enhancer-dependent gene activation
a–c, Scatter plots showing the changes of H3K27ac (a) and H3K4me1 (b) ChIP-seq signal levels upon CTCF loss on all significant peak regions in ESCs (left) and NPCs (right). The changes of H3K4me3 ChIP-seq signal levels on all peak regions on transcription start sites (TSSs) are also shown (c). d, Boxplots showing the changes of H3K27ac (top) and H3K4me1 (bottom) ChIP-seq signal levels on distal element loci of all analyzed E-P contacts. The changes upon CTCF depletion in ESC (left) and NPC stage (middle) and the changes during neural differentiation (right) are shown. The numbers of data points are also indicated on the bottom. NS not significant, *** p value < 0.001, two-tailed t-test.
