Extended Data Fig. 6: Characterization of the proteome of mutant mice.
From: Imbalances in the eye lens proteome are linked to cataract formation
(a) Left, body weight (filled symbols) and lens wet weight (open symbols), per pair of eye lenses from one individual: αA mutant (squares), βA2 mutant (circles) and γD mutant (triangles). Middle and right, amount of water-soluble and -insoluble protein was determined by the Bradford protein assay using BSA as a standard. Data are given as mean ± SD of biological replicates (animals or pairs of eye lenses; please see Supplementary Table 2 and 3 for exact sample size). (b) Top, 2-DE map of the water-soluble fraction of lenses from newborn wild-type mice. Bottom, densitometric analysis; only the low molecular crystallin region was considered (see excerpt). The fraction of α- (gold), β- (orange) and γ-crystallins (blue) was determined up to 12 months of age, for the water-soluble (solid bars) and -insoluble (shaded bars) fraction. Please see Supplementary Data 1 for numerical values.
