Extended Data Fig. 4: 2-DE of crystallin fractions from wild-type and mutant mice.
From: Imbalances in the eye lens proteome are linked to cataract formation
After separation by SEC-HPLC, the individual fractions were checked for their protein composition using 2-DE. Note that the type of ampholyte used (Pharmalyte) caused a staining artifact but that did not influence IEF or second dimension separation of the proteins, as shown at the bottom right. For densitometric analysis, the background signal of 2-DE gels was subtracted using the rolling ball background correction with 100 px in ImageJ. For each individual fraction, one 2-DE experiment was performed.
