Extended Data Fig. 8: ICL2 interaction with a hydrophobic pocket of Gi.
From: Cryo-EM structure of an activated GPCR–G protein complex in lipid nanodiscs
a, Structure of GDP-Gαi showing a hydrophobic network surrounding F336 in the zoomed-in view. Residues involved in the network are shown as sticks. b, Atomic model of C-NTS-NTSR1-Gi-cND showing insertion of F17534.51 from ICL2 of NTSR1 into a hydrophobic pocket involving residues F336, L194 and V339 of Gαi. Residues involved in the network are shown as sticks. Residues from the network in (a) are shown in lines. A transition of F336 on Gαi from the network in (a) in the GDP-bound state to a new network in (b) in the NTSR1-bound state is observed.
